Semitruck involved in I90 crash was carrying missiles

first_imgSPOKANE — The Idaho State Police say a semi-truck that crashed on Interstate 90 on Friday night was carrying 16 2,000-pound missiles.The Spokesman Review reports the 47-year-old driver, Mark W. Dearinger of Chickasha, Oklahoma, was cited for inattentive driving.He was not injured in the crash.The state police say Dearinger drove through an eastbound Idaho Transportation Department checkpoint at about 7:15 p.m. Friday and drove into a hazmat containment area by accident instead of merging back on to I-90. He drove to the end of the containment area and over a large snow bank, disabling the truck.Fairchild Air Force Base assisted emergency personnel by sending three explosive ordnance disposal technicians to the scene, although the semi-truck involved did not have a direct relationship with the base.Multiple attempts to contact Fairchild for more information went unanswered Saturday.last_img read more

Read More
UBM Completes Canon Deal Says Print Sales Continue Decline

first_imgUBM also reported that year-to-date profits for its events unit are up more than 20 percent to roughly $94.2 million while its data service and online business saw profits slide 7.1 percent to about $32.7 million. Profits for its targeting, distribution and monitoring business stayed flat at about $49.1 million.Overall, UBM’s total adjusted operating profits through the first nine months grew 8 percent to $172.5 million. London-based United Business Media says it has completed its $287 million acquisition of medical industry publisher and event producer Canon Communications. The deal agreement was announced mid-September.UBM also has released an interim management statement detailing the company’s performance through the third quarter. Print sales, the company says, are down 15.3 percent to approximately $163.7 million over the nine-month period. That’s an underlying decline of 7.2 percent, adjusted for discontinued publications. Although UBM did not shutter any print titles during the third quarter it has ceased publishing at least 15 magazines since September 2009. Meanwhile, adjusted operating profit for its print magazines grew to about $5.6 million from $2 million last year.In the statement, UBM says it will continue to actively manage its print portfolio. A UBM spokesperson did not immediately return a request seeking comment concerning the company’s future plans for the 24 print titles it added as a result of the Canon acquisition.last_img read more

Read More
GoFundMe Page Created For Wilmington Native In Need Of Wheelchair Accessible Van

first_imgWILMINGTON, MA — Wilmington native Kristen Curran Gannon recently battled an acute life-threatening heart condition. She had an ascending and descending aortic dissection. During one of her surgeries, Kristen suffered irreversible damage to her lower organs and her intestines ruptured, causing a stroke in her spine.Doctors told Kristen that she would no longer have use of her legs and would live the rest of her life in a wheelchair.  She was subsequently diagnosed with Marfan Syndrome, a genetic connective tissue disease that has made it extremely difficult for her to heal and recover.Kristen’s parents are hoping to purchase a wheelchair accessible van with a ramp for Kristen. Kristen’s mom Elaine Curran, was a resident of Wilmington for almost 60 years before moving to New Hampshire. She taught school in Wilmington at the Wildwood and the North Intermediate for almost 40 years before retirement.Kristen’s friends has created a GoFundMe page to assist the family with the purchase. The page reads, in part:Our family has never asked for help and it is not something we are completely comfortable with even now. But, so many people have asked how they can help so we thought if we were able to get help funding a new wheelchair accessible van for Kristen, we could ALL share in something that would have unlimited potential to improve the quality of Kristen’s life as well as our parents who continue to sacrifice every day because they love her so much.Make a donation to the GoFundMe page HERE.Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedGoFundMe Page Created For Wilmington Family With 14-Year-Old Battling CancerIn “Community”CEILI STRONG: Music Bingo Fundraiser In Memory Of Ceili Kinneen Set For Tremezzo On October 24In “Community”5 Things To Do In Wilmington On Friday, August 30, 2019In “5 Things To Do Today”last_img read more

Read More
HTC shows off the Vive Cosmos VR headset

first_img Now playing: Watch this: Comment Share your voice VR games you need to start playing right away 1 HTC Vive See It VR games on CNET Preview • Here’s what it’s like to use the HTC Vive, the $799 VR headset that you can preorder today Mentioned Above HTC Vive CNET may get a commission from retail offers. 9 Photos $499 $689 Walmart 1:47 See it Review • HTC Vive review: Yes, this is the best VR experience, if you’ve got the space The Vive Cosmos has six cameras. HTC HTC has unveiled the look of its next Vive virtual reality headset, with the Cosmos to have a flip-up design, six cameras, detachable headphones, a faceplate and a vented front. Billed as a premium PC VR system, the Cosmos, first teased at CES 2019 in Las Vegas in January, will have “striking graphics [and] lifelike sound,” HTC said Friday. HTC Vive Pro Eye tracks your eyes with pinpoint accuracy,… Mobile Gadgets Gaming Accessories Gaming Virtual Reality Apps Up close with the HTC Vive Pro Tags The Cosmos will have a “pixel-packed” display that minimizes the screen-door effect of using VR systems, HTC said. The company also touted its new tracking system.”With wide and accurate tracking, gesture controls and a six-degrees-of-freedom (6DoF) headset and controller setup, Vive Cosmos promises a deeply engaging VR experience,” HTC said.htc-vive-cosmos-vr-headset HTC The system can be used straight out of the box with minimal setup and also features a more comfortable headset with soft, light and breathable material, HTC said. The company also unveiled new Vive controllers that it called gamer friendly, versatile and practical.htc-vive-cosmos-controllerThe new Vive Cosmos controllers. HTC HTC said it’ll reveal the official specs next week.HTC’s Viveport Infinity unlimited subscription service launched April 2, with subscriptions costing $13 a month or $99 for an entire year. The service lets customers download more than 600 VR games and apps through the Viveport store. This morning we unveiled the HTC VIVE COSMOS, with 6 cameras, a flip up design, detachable headphones, faceplate & vented front. Next week, we’ll get into specs. Stay tuned. https://t.co/TSCJVM9eLs #HTCVIVE #HTCVIVECOSMOS— HTC VIVE (@htcvive) June 21, 2019 HTC had first looked to end the drought of VR content by launching the Viveport subscription service back in 2017, giving Vive headset owners up to five titles at a time for $9 a month. It then expanded the service in August 2018 to support rival Oculus Rift headsets. HTClast_img read more

Read More
Nanoscale magnetic imaging of ferritin in a single cell

first_img This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. In the present work, Wang et al. reported two technical advancements to allow nanoscale MI of intracellular proteins within a single cell. For this, they freeze-fixed the cell to a solid state and intricately segmented it to a cube shape, then placed it on a tuning fork scanning probe of an atomic force microscope (AFM) for imaging, where the flat cross section of the cell was exposed to air. The scientists used the sample placement setup to allow the NV sensor to be positioned within 10 nm of the target proteins and used the AFM to suppress thermal drift during sample positioning. They then engineered trapezoidal cylinder-shaped nanopillars at a bulk diamond surface for image acquisition, technically shortening the time of image acquisition by one order compared to previous methods. In the present study, the scientists used this technique to conduct in situ MI of the magnetic fluctuating noise of intracellular ferritin proteins (a biomarker of iron stores and transferrin saturation in the body) within the experimental setup. In life sciences, the ability to measure the distribution of biomolecules inside a cell in situ is an important investigative goal. Among a variety of techniques, scientists have used magnetic imaging (MI) based on the nitrogen vacancy center (NV) in diamonds as a powerful tool in biomolecular research. However, nanoscale imaging of intracellular proteins has remained a challenge thus far. In a recent study now published in Science Advances, Pengfei Wang and colleagues at the interdisciplinary departments of physics, biomacromolecules, quantum information and life sciences in China, used ferritin proteins to demonstrate the MI realization of endogenous proteins in a single cell, using the nitrogen-vacancy (NV) center as the sensor. They imaged intracellular ferritins and ferritin-containing organelles using MI and correlative electron microscopy to pave the way for nanoscale magnetic imaging (MI) of intracellular proteins. The scientists measured fluorescence decay at a fixed free evolution time of 50 microseconds (τ = 50 μs) to reveal the degree of NV sensor spin polarization, which correlated with the amount of ferritin in the sensing volume. They observed the appearance of some clusters via both TEM and MI images, although some details were not observed in MI, the results confirmed that spin noise from intracellular ferritin contributed to depolarize the NV center. In order to obtain details of the ferritin clusters at higher resolution, the scientists minimized the pixel size to 8.3 nm and acquired MI of high resolution of the proteins as expected. In this way, Wang et al. explored the sensitivity of NV centers as an appropriate sensor for biological imaging applications at the level of the single molecule. They used the technique as a sensor in the experimental setup to obtain the first MI of a protein at a resolution of 10 nm in situ. The scientists aim to improve the stability and sensitivity of the technique to speed up the scanning process and image a larger area of interest in the cell and locate ferritin beyond the nucleus in association with additional organelles. New method discovered to view proteins inside human cells Explore further Ferritin is a globular protein complex with an outer diameter of 12 nm, containing a cavity spanning 8 nm in diameter that allows up to 4500 iron atoms to be stored within the protein. The magnetic noise of the ferric ions can be detected due to their effects on the T1 relaxation time of an NV center. In this work, Wang et al. confirmed the observation using fluorescence measurements of time-dependent decay of the population of NV centers (magnetic spin, mS = 0 state), in a diamond surface coated with ferritins. Additionally, the scientists detected the magnetic noise with label-free methods using the NV center via transmission electron microscopy (TEM). The work allowed the development of a correlated MI and TEM scheme to obtain and verify the first nanoscale MI of a protein in situ. The scientists used the hepatic carcinoma cell line (HepG2) for the experiments and studied iron metabolism by treating the cells with ferric ammonium citrate (FAC), which significantly increased the amount of intracellular ferritin. They verified this using confocal microscopy (CFM), western blotting and TEM techniques at first. The results showed the primary localization of ferritins in the intracellular puncta around the nucleus, among the cytoplasm. The scientists used bulk electron paramagnetic resonance (EPR) spectroscopy to confirm the paramagnetic properties of ferritin in the FAC-treated HepG2 cells and mass spectroscopy to measure the interference due to other paramagnetic metal ions. Journal information: Science Advances © 2019 Science X Network Increasing existing spatial resolution of biomedical imaging is required to achieve ongoing demands in medical imaging, and therefore, among a variety of techniques, magnetic imaging is of broad interest at present. Magnetic resonance imaging (MRI) is widely used to quantify the distribution of nuclear spins but conventional MRI can only reach a resolution of 1 µm in nuclear spin imaging where the resolution is limited by electrical detection sensitivity. Scientists have developed a series of techniques to break this resolution barrier, including a superconducting quantum interference device and magnetic resonance force microscopy. Nevertheless, these reports require a cryogenic environment and high vacuum for imaging, limiting the experimental implementation and its translation to clinical practice. A recently developed quantum sensing method based on the nitrogen vacancy center in diamond has radically pushed the boundary of MI techniques at the nanoscale to detect organic molecules and proteins in the lab. Scientists have combined quantum sensing with NV centers and scanning probe microscopy to demonstrate nanoscale MRI for single electron spin and small nuclear spin ensemble while using the NV center as a biocompatible magnetometer to noninvasively image ferromagnetic particles within cells at the subcellular scale (0.4 µm). For example, depolarization of the NV center can be used as a wideband magnetometer to detect and measure fluctuating noise from metal ions and nuclear spins. However, such imaging of single proteins via MI at the nanoscale has not been reported in the single cell thus far. Citation: Nanoscale magnetic imaging of ferritin in a single cell (2019, April 18) retrieved 18 August 2019 from https://phys.org/news/2019-04-nanoscale-magnetic-imaging-ferritin-cell.html Correlative MI and TEM images. (A) Schematic view of sectioning for correlative MI and TEM imaging. The last section and the remaining cube were transferred for TEM imaging and MI scanning, respectively. The sectioning resulted in some split ferritin clusters that could be imaged under both microscopes. A transparent blue strip of ~10 nm indicates the imaging depth of the MI, while in the TEM, the imaging depth is ~100 nm. (B) Distribution of ferritins from the last ultrathin section under TEM. Inset: Magnified figure of the part in black dashed box. (C) MI result of the remaining cell cube. The pixel size is 43 nm. (D) The merged MI and TEM micrograph shows ferritins in a membrane-bound organelle. The red arrows in (B) to (D) indicate the same ferritin cluster. Scale bars, 5 μm (B) and 1 μm [B (inset), C, and D]. Credit: Science Advances, doi: 10.1126/sciadv.aau8038.center_img TOP – The preparation and characterization of ferritin-rich HepG2 cell samples. (A) Schematic view of the treatment to cultured cells. Following iron loading or no treatment, the HepG2 cells were examined for fluorescence images and EPR spectra, respectively. For the MI and TEM imaging, cell samples were treated through high-pressure freezing, freeze substitution, and sectioning. (B) Representative confocal microscopy (CFM) image of ferritin structures (green) in iron-loaded HepG2 cells. The ferritin proteins were immunostained by anti-ferritin light chain antibody. The nuclei are indicated by 4′,6-diamidino-2-phenylindole (DAPI) in the blue channel. Inset displays magnified ferritin structures. The yellow dashed line outlines the contour of a cell. Scale bar, 20 μm. (C) EPR spectra of control and iron-loaded HepG2 cells at T = 300 K. BOTTOM – Adjusting the distance between the NV center and the cell section. (A) Interference fringes between the cell cube and the diamond surface. Scale bar, 20 μm. (B) The geometric relation and the gap R between cell samples and diamond-pillars for MI. The top surface diameter of the nanopillar is 400 nm. Credit: Science Advances, doi: 10.1126/sciadv.aau8038. The work will contribute to clinical diagnostics to determine biomarker-based iron storage and release in cells. This will include studies on the regulatory mechanisms of iron metabolism during the progression of hemochromatosis, anemia, liver cirrhosis and Alzheimer’s disease. Wang et al. propose to extend the approach in situ to other cellular components with paramagnetic signals, including magnetic molecules, metalloproteins and special spin-labelled proteins. The scientists envision that further studies will explore additional targets suitable for high-resolution MI and correlated TEM imaging techniques, with optical microscopy detection incorporated to the experimental setup to extend the work and determine protein nuclear spin MRI as well as perform three-dimensional cell tomography. More information: Mamin H.J. et al. February 2013, Science. Pengfei Wang et al. Nanoscale magnetic imaging of ferritins in a single cell, Science Advances (2019). DOI: 10.1126/sciadv.aau8038 Denis Vasyukov et al. A scanning superconducting quantum interference device with single electron spin sensitivity, Nature Nanotechnology (2013). DOI: 10.1038/nnano.2013.169 D. Rugar et al. Single spin detection by magnetic resonance force microscopy, Nature (2004). DOI: 10.1038/nature02658H. J. Mamin et al. Nanoscale Nuclear Magnetic Resonance with a Nitrogen-Vacancy Spin Sensor, Science (2013). DOI: 10.1126/science.1231540 , Nature Nanotechnology Wang et al. then used ultrafast, high-pressure freezing to immobilize all intracellular components of the Fe-loaded cells. The process stabilized the intracellular structures and molecules by minimizing Brownian motion in cells, which typically contributes to random motion of proteins up to 100 nm in vivo. To image the samples, they embedded and polymerized the frozen cells in LR White medium, followed by gluing the embedded cell sample to the AFM tuning fork with a few cells at the tip. Using a diamond knife, the scientists then sectioned the tip surface to nanometer flatness to examine the cuboid cell section under AFM. They acquired MI images of ferritins by scanning the cell cube along the diamond nanopillars and simultaneously measured NV spin repolarization rate using the “leapfrog” scanning mode of the microscope as detailed previously. , Science , Nature Schematic of the setup and experimental principle. (A) Schematic view of the experimental setup. The cell embedded in resin is attached to a tuning fork and scans above the diamond nanopillar that contains a shallow NV center. A copper wire is used to deliver the microwave pulse to the NV center. A green laser (532 nm) from the confocal microscope (CFM) is used to address, initialize, and read out the NV center. (B) Left: Crystal lattice and energy level of the NV center. The NV center is a point defect that consists of a substitutional nitrogen atom and an adjacent vacancy in diamond. Right: Schematic view of a ferritin. The black arrows indicate the electron spins of Fe3+. (C) Experimental demonstration of the spin noise detection with and without ferritin in the form of polarization decay for the same NV center. The inset is the pulse sequence for detection and imaging of the ferritin. A 5-μs green laser is used to initialize the spin state to ms = 0, followed by a free evolution time τ to accumulate the magnetic noise, and finally the spin state is read out by detecting the fluorescence intensity. The pulse sequence is repeated about 105 times to acquire a good signal-to-noise ratio (SNR). The relaxation time is fitted to be 0.1 and 3.3 ms by exponential decay for the case with and without ferritin, respectively, indicating a spin noise of 0.01 mT2. Credit: Science Advances, doi: 10.1126/sciadv.aau8038. LEFT – Experimental setup. The experiment was carried out on a homebuilt setup, which combined optically detected magnetic resonance microscopy (ODMR) with atomic force microscopy (AFM). DM: dichroic mirror. BP: bandpass filter working at 650–775 nm. APD: avalanche photodiode. CCD: charge coupled device. LED: light emitting diode of 470 nm. AL: achromatic lens. PH: pinhole at a size of 30 μm. BS: beam splitter. RIGHT – Images of the nanopillars on diamonds. (A) SEM imaging of the fabricated diamond nanopillars just after reactive ion etching (RIE). The top of the nanopillar is covered by the hydrogen silsesquioxane (HSQ) to protect the NV center. (B) A single trapezoidal-cylinder shaped nanopillar to sense cell sections adhered at the AFM tip. Scale bars, 10 μm (A); 400 nm (B). Credit: Science Advances, doi: 10.1126/sciadv.aau8038. (A) Ferritin cluster imaged by the NV sensor with 80 × 24 pixels and a pixel size of 8.3 nm. Scale bar, 100 nm. (B) Trace data of the scanning line in (A) directed by the red arrow. The platform indicates the ferritin cluster. The red curve fitted by a plateau function serves as a guide to the eye. (C) Magnified figure of the gold dashed box in (B). The sharp transition indicated by the red arrow around x = 283 nm shows the scanning from the blank area to the area with ferritins. Credit: Science Advances, doi: 10.1126/sciadv.aau8038.last_img read more

Read More
Rousseff reelected on call to save Brazils social gains

first_imgIn a campaign that featured ‘change’ as the buzzword for both sides, the incumbent argued that Neves’s economic proposals would produce recession and erase gains for 36 million Brazilians who have risen from extreme poverty under her party’s rule. Rousseff in her victory speech pledged to engage in dialog as she vows to stimulate economic growth and fight corruption. ‘Some times in history, narrow results produced much stronger changes than very wide victories,’ she said in Brasilia. ‘From now on in Brazil we will have a debate of ideas, clash of positions that may produce areas of consensus capable of moving our society along the paths of change that we so badly need. My first words are a call for peace and unity.’ Neves said in his concession speech that he phoned Rousseff to casking to unite the country.last_img read more

Read More
Holland Americas Explore4 deal includes up to US2000 in EBBs

first_img Michael Smith Posted by << Previous PostNext Post >> Holland America’s Explore4 deal includes up to US$2,000 in EBBs Tags: Holland America Linecenter_img Thursday, July 28, 2016 SEATTLE — Holland America Line has brought back Explore4 with four promotions on select January 2017 – April 2018 sailings.Clients looking to book a cruise or Land+Sea Journey for 2017 and beyond can take advantage of complimentary beverages and specialty dining aboard ship, as well as deposits reduced by 50% and fares for friends and family. Suite bookings feature an Internet credit and prepaid gratuities.The Explore4 promotion for guests booking any category stateroom includes a Signature Beverage Package valued at up to US$1,400, dinner in the Pinnacle Grill, reduced cruise fares for friends and kids in the same stateroom and 50% reduced deposits. Bookings must be made by Nov. 18, 2016.Clients who book a cruise in a suite category stateroom will receive the above four offers plus an additional $200 Internet credit per stateroom ($100 per person) and prepaid gratuities (valued at $13.50 per guest, per day).“Cruisers are early planners, and our popular Explore4 promotion encourages our guests to book their vacation now while rewarding them with some exceptional bonuses,” said Orlando Ashford, president of Holland America Line. “By making their reservations during Explore4 guests have more itinerary and stateroom options for next year, and the value of a cruise vacation increases even more.”More news:  ‘Turn around year’ for TPI brings double-digit growthPassengers who book any category stateroom receive a Signature Beverage Package valued at up to $1,400 per stateroom ($700 per person) that includes wine, beer, spirits, cocktails, sodas and coffee. The beverage offer is only applicable on the cruise portion of Alaska Land+Sea Journeys and the package value is based on a 14-day cruise and increases based on days if a longer cruise is booked.Explore4 offers also include a free dinner at the Pinnacle Grill. Plus there are reduced cruise fares on select sailings for friends and kids sharing a stateroom with two other guests.In the Caribbean, fares for seven-day cruises start from $799 for the first and second guests in an oceanview stateroom and reduced fares apply for the third and fourth guests. A seven-day European cruise starts at $999 for an oceanview stateroom for the first and second guests, with reduced fares for third and fourth guests.Explore4 covers select itineraries to nearly all of Holland America Line’s global destinations, including Alaska and Alaska/Yukon Land+Sea Journeys, Asia, Australia/New Zealand, Baltic, Bermuda, Canada/New England, Caribbean, Europe, Hawaii, Mediterranean, Mexico, Panama Canal and South America. Sharelast_img read more

Read More